The general service area of the Mon valley Regional Chamber is the Mid Mon Valley region of Southwestern Pennsylvania; however, membership is open to all businesses.
- Allenport
- Belle Vernon
- Bentleyville
- California
- Caroll Township
- Charleroi
- Donora
- Dunlevy
- Elco
- Fallowfield Township
- Fayette County
- Finleyville
- Forward Township
- Fredericktown
- Jefferson Township
- Long Branch
- Monessen
- Monogahela
- New Eagle
- Newell
- North Belle Vernon
- North Carolina
- Roscoe
- Rostraver Township
- Smithton
- Speers
- Stockdale
- Twilight
- Union Township
- Washington Township
- West Brownsville
- West Newton
- West Pike Run Township